1. The solid surface on which cells grow: This is the most common usage. In this context, the substrate is typically a culture dish, flask, or plate made of a material like polystyrene or glass . These surfaces are often treated with adhesion factors like collagen, fibronectin, or laminin , which promote cell attachment and spreading.
2. The nutrients and factors that cells need to survive and proliferate: This is a broader definition and encompasses the culture medium , which is a liquid solution containing:
* Essential nutrients: sugars, amino acids, vitamins, and salts
* Facteurs de croissance: proteins that stimulate cell growth and division
* Buffers: maintain the pH of the medium
* Antibiotiques: prevent bacterial contamination
Par conséquent, le "substrat" pour les cellules de la culture cellulaire peut être à la fois la surface solide sur laquelle ils se développent et le milieu liquide qui leur fournit des nutriments et des facteurs essentiels.
